https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37384 Bacillus licheniformis strain KX657843 based on 16S rRNA sequencing and phylogenetic analysis, was isolated from earthworms (Metaphire posthuma). The bacteria was found to be uniquely influenced by the amino acid L-asparagine monohydrate. The organism produced the maximum yield of 2.7 g L⁻¹ EPS in the presence of sucrose supplemented with L-asparagine monohydrate while no EPS was produced in absence of the amino acid. The order of growth and EPS production in presence of different carbon substrates supplemented with L-asparagine monohydrate was observed as sucrose > glucose > glycerol > mannitol > citrate > cellulose > starch. L-asparagine monohydrate could serve as the sole nitrogen source for the organism but it alone cannot satisfy the requisite carbon demand for growth and EPS production. EPS production showed a positive correlation with sucrose concentration but a negative correlation with citrate levels. Analysis of extracted EPS using Fourier transform infrared spectroscopy and measurement of its zeta potential revealed the chemical composition and anionic nature of the EPS. The bacterium produced 119.235 IU mL⁻¹ extracellular L-asparaginase. This is the first report of an L-asparagine monohydrate dependent EPS producing Bacillus licheniformis from the gut of the earthworm, Metaphire posthuma.]]> Wed 28 Oct 2020 18:05:03 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:54047 10 μg/L was much greater in the regions between two major rivers than in the regions close to the Ganges River on the eastern border of the study area, where higher proportions of As concentrations >10 μg/L had been observed prior to 2005. The greater likelihood that toxic concentrations of As are present away from the river channel and is found instead in the interfluvial regions could be attributed to the transport and flushing of aquifer As from intense irrigation pumping. We estimated that about 2.8 million people could be chronically exposed to As concentrations >10 μg/L. This high population-level exposure to elevated As concentrations could be reduced through targeted well-testing campaigns, promoting well-switching, provisions for safe water access, and developing plans for raising public awareness. Policymakers could use the ternary hazard map presented here to target high-risk localities for priority implementation of piped water supply strategies to help reduce human suffering.]]> Tue 30 Jan 2024 13:50:01 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:38889 Thu 24 Feb 2022 16:26:28 AEDT ]]>